EP 1232278 A1 20020821 - MICROBIAL 9$g(a)-HYDROXYLATION OF STEROIDS
Title (en)
MICROBIAL 9$g(a)-HYDROXYLATION OF STEROIDS
Title (de)
9ALPHA-HYDROXYLIERUNG VON STEROIDEN IN MIKROORGANISMEN
Title (fr)
9$G(a)-HYDROXYLATION MICROBIENNE DE STEROIDES
Publication
Application
Priority
- EP 00977417 A 20001017
- EP 0010223 W 20001017
- EP 99203470 A 19991022
- EP 99204449 A 19991222
Abstract (en)
[origin: WO0131050A1] A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein multiple genes involved in steroid nucleus degradation are inactivated, such as steroid dehydrogenase genes. Examples for such genes are kstD1 and kstD2: strains with multiple inactivated steroid degrading enzyme genes can be used in the accumulation of steroid intermediates with a high yield. A preferred accumulation product is 9 alpha -hydroxy-4-androstene-3,17-dione.
IPC 1-7
C12P 33/00; C12N 9/02; C12N 15/53; C12N 15/63; C12N 15/01; C12N 1/21; C12N 15/76
IPC 8 full level
C12N 1/21 (2006.01); C12N 9/02 (2006.01); C12N 15/01 (2006.01); C12N 15/53 (2006.01); C12N 15/63 (2006.01); C12N 15/76 (2006.01); C12P 33/00 (2006.01); C12P 33/06 (2006.01)
CPC (source: EP)
C12N 1/205 (2021.05); C12N 9/001 (2013.01); C12P 33/06 (2013.01); C12R 2001/01 (2021.05)
Citation (search report)
See references of WO 0131050A1
Citation (examination)
- KIESLICH (1985) J. Basic Microbiol. 25, 461-474.
- WOVCHA ET AL. (1979) Biochim. Biophys. Acta 574, 471-479.
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 0131050 A1 20010503; AR 026186 A1 20030129; AU 1514501 A 20010508; AU 775476 B2 20040805; CA 2396879 A1 20010503; CN 1224716 C 20051026; CN 1413260 A 20030423; CZ 20021784 A3 20020814; EP 1232278 A1 20020821; IL 149715 A0 20021110; NO 20022449 D0 20020523; NO 20022449 L 20020619; NZ 519039 A 20040924; RU 2002113373 A 20040227; RU 2268935 C2 20060127
DOCDB simple family (application)
EP 0010223 W 20001017; AR P000105523 A 20001020; AU 1514501 A 20001017; CA 2396879 A 20001017; CN 00817567 A 20001017; CZ 20021784 A 20001017; EP 00977417 A 20001017; IL 14971500 A 20001017; NO 20022449 A 20020523; NZ 51903900 A 20001017; RU 2002113373 A 20001017