EP 1278876 A2 20030129 - STABLE i ZYMOMONAS MOBILIS /i XYLOSE AND ARABINOSE FERMENTING STRAINS

Title (en)

STABLE i ZYMOMONAS MOBILIS /i XYLOSE AND ARABINOSE FERMENTING STRAINS

Title (de)

STABILE XYLOSE- UND ARABINOSEVERGÄRENDE ZYMOMONAS MOBILIS STÄMME

Title (fr)

SOUCHES DE $I(ZYMOMONAS MOBILIS) STABLES INDUISANT LA FERMENTATION DU XYLOSE ET DE L'ARABINOSE

Publication

EP 1278876 A2 20030129 (EN)

Application

EP 01923213 A 20010406

Priority

CA 2304929 A 20000502
US 0111334 W 20010406
US 56523300 A 20000501

Abstract (en)

[origin: WO0183786A2] The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt, at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transfomed, is also provided. The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.

IPC 1-7

C12N 15/74; C12N 15/90; C12N 15/52; C12N 1/21; C12P 7/06

IPC 8 full level

C12N 15/09 (2006.01); C12N 1/21 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/24 (2006.01); C12N 9/92 (2006.01); C12N 15/74 (2006.01); C12N 15/90 (2006.01); C12P 7/06 (2006.01); C12R 1/01 (2006.01)

CPC (source: EP)

C12N 9/1022 (2013.01); C12N 9/1205 (2013.01); C12N 9/92 (2013.01); C12N 15/74 (2013.01); C12N 15/90 (2013.01); C12N 15/902 (2013.01); C12P 7/06 (2013.01); Y02E 50/10 (2013.01)

Citation (search report)

See references of WO 0183786A2

Citation (examination)

DE LORENZO V ET AL: "MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS PROMOTER PROBING AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA", JOURNAL OF BACTERIOLOGY, vol. 172, no. 11, 1990, pages 6568 - 6572, ISSN: 0021-9193
ANONYMOUS: "Office of Fuels Development Project Summaries Fiscal Years 96 & 97 Biofuels Program", 1998, Retrieved from the Internet <URL:http://www.p2pays.org/ref/17/16271/bioprogram.html> [retrieved on 20080507]
HERRERO M ET AL: "TRANSPOSON VECTORS CONTAINING NON-ANTIBIOTIC RESISTANCE SELECTION MARKERS FOR CLONING AND STABLE CHROMOSOMAL INSERTION OF FOREIGN GENES IN GRAM-NEGATIVE BACTERIA", JOURNAL OF BACTERIOLOGY, vol. 172, no. 11, 1990, pages 6557 - 6567, ISSN: 0021-9193

Designated contracting state (EPC)

AT BE CH CY DE FI FR GB LI SE

DOCDB simple family (publication)

WO 0183786 A2 20011108; WO 0183786 A3 20020620; WO 0183786 B1 20020718; AU 2001249926 B2 20060720; AU 4992601 A 20011112; BR 0110987 A 20060207; BR 0110987 B1 20140715; EP 1278876 A2 20030129; JP 2004512819 A 20040430; JP 4510355 B2 20100721

DOCDB simple family (application)

US 0111334 W 20010406; AU 2001249926 A 20010406; AU 4992601 A 20010406; BR 0110987 A 20010406; EP 01923213 A 20010406; JP 2001580393 A 20010406