EP 1292707 A2 20030319 - OLIGONUCLEOTIDES OR PNA OLIGOMERS AND A METHOD FOR DETECTING THE METHYLATION STATE OF GENOMIC DNA IN A PARALLEL MANNER
Title (en)
OLIGONUCLEOTIDES OR PNA OLIGOMERS AND A METHOD FOR DETECTING THE METHYLATION STATE OF GENOMIC DNA IN A PARALLEL MANNER
Title (de)
OLIGONUKLEOTIDE ODER PNA-OLIGOMERE UND VERFAHREN ZUR PARALLELEN DETEKTION DES METHYLIERUNGSZUSTANDES GENOMISCHER DNA
Title (fr)
OLIGONUCLEOTIDES OU OLIGOMERES DE PNA, ET PROCEDE DE DETECTION PARALLELE DE L'ETAT DE METHYLATION D'UN ADN GENOMIQUE
Publication
Application
Priority
- DE 0101089 W 20010315
- DE 10013847 A 20000315
Abstract (en)
[origin: DE10013847A1] Parallel detection of methylation in genomic DNA comprising: (a) chemically treating a DNA sample so that 5' unmethylated cytosine is converted to uracil/thymidine; (b) amplifying the sample up to 10 different fragments, each <= 2000 base pairs (bp) using synthetic primers; (c) hybridizing the amplified sequence to a an oligonucleotide or PNA-oligomer containing at least two sequences fully defined in the specification; and (d) detecting hybridization, is new. An Independent claim is also included for oligonucleotides or PNA-oligomers for detection of cytosine methylation in chemically treated genomic DNA comprising one of the sequences fully defined in the specification
IPC 1-7
IPC 8 full level
C07K 14/47 (2006.01); C07K 14/82 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6853 (2018.01)
CPC (source: EP US)
C07K 14/4703 (2013.01 - EP US); C07K 14/82 (2013.01 - EP US); C12Q 1/6837 (2013.01 - EP US); C12Q 1/6853 (2013.01 - EP US)
C-Set (source: EP US)
Citation (search report)
See references of WO 0168910A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
DE 10013847 A1 20010927; AU 5460101 A 20010924; EP 1292707 A2 20030319; US 2005202420 A1 20050915; WO 0168910 A2 20010920; WO 0168910 A3 20030103
DOCDB simple family (application)
DE 10013847 A 20000315; AU 5460101 A 20010315; DE 0101089 W 20010315; EP 01927582 A 20010315; US 22187802 A 20020916