Title (en)

OLIGONUCLEOTIDES OR PNA OLIGOMERS AND A METHOD FOR DETECTING THE METHYLATION STATE OF GENOMIC DNA IN A PARALLEL MANNER

Title (de)

OLIGONUKLEOTIDE ODER PNA-OLIGOMERE UND VERFAHREN ZUR PARALLELEN DETEKTION DES METHYLIERUNGSZUSTANDES GENOMISCHER DNA

Title (fr)

OLIGONUCLEOTIDES OU OLIGOMERES DE PNA, ET PROCEDE DE DETECTION PARALLELE DE L'ETAT DE METHYLATION D'UN ADN GENOMIQUE

Publication

EP 1292707 A2 20030319 (DE)

Application

EP 01927582 A 20010315

Priority

• DE 0101089 W 20010315
• DE 10013847 A 20000315

Abstract (en)

[origin: DE10013847A1] Parallel detection of methylation in genomic DNA comprising: (a) chemically treating a DNA sample so that 5' unmethylated cytosine is converted to uracil/thymidine; (b) amplifying the sample up to 10 different fragments, each <= 2000 base pairs (bp) using synthetic primers; (c) hybridizing the amplified sequence to a an oligonucleotide or PNA-oligomer containing at least two sequences fully defined in the specification; and (d) detecting hybridization, is new. An Independent claim is also included for oligonucleotides or PNA-oligomers for detection of cytosine methylation in chemically treated genomic DNA comprising one of the sequences fully defined in the specification

IPC 1-7

C12Q 1/68

IPC 8 full level

C07K 14/47 (2006.01); C07K 14/82 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6853 (2018.01)

CPC (source: EP US)

C07K 14/4703 (2013.01 - EP US); C07K 14/82 (2013.01 - EP US); C12Q 1/6837 (2013.01 - EP US); C12Q 1/6853 (2013.01 - EP US)

Citation (search report)

See references of WO 0168910A2

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

DE 10013847 A1 20010927; AU 5460101 A 20010924; EP 1292707 A2 20030319; US 2005202420 A1 20050915; WO 0168910 A2 20010920; WO 0168910 A3 20030103

DOCDB simple family (application)

DE 10013847 A 20000315; AU 5460101 A 20010315; DE 0101089 W 20010315; EP 01927582 A 20010315; US 22187802 A 20020916