EP 1294926 A2 20030326 - TEST SYSTEM FOR DETERMINING THE ACTIVITY OF CYCLO-NUCLEOTIDE-DEPENDENT PROTEIN KINASES AND VASP PHOSPHATASES
Title (en)
TEST SYSTEM FOR DETERMINING THE ACTIVITY OF CYCLO-NUCLEOTIDE-DEPENDENT PROTEIN KINASES AND VASP PHOSPHATASES
Title (de)
TESTSYSTEM ZUR BESTIMMUNG DER AKTIVITÄT VON CYCLO-NUKLEOTID-ABHÄNGIGEN PROTEINKINASEN UND VASP-PHOSPHATASEN
Title (fr)
SYSTEME DE TEST POUR DETERMINER L'ACTIVITE DE PROTEINES KINASES DEPENDANTES DES CYCLONUCLEOTIDES ET DE VASP-PHOSPHATASES
Publication
Application
Priority
- DE 10029210 A 20000614
- EP 0106621 W 20010612
Abstract (en)
[origin: WO0196594A2] The invention relates to an HTS-appropriate test system for detecting the activity of cyclo-nucleotide-dependent protein kinases (cNPK) containing: a) at least one test compound; b) at least one appropriate cNPK substrate; c) at least one composition, which is to be incubated and which contains cNPK and ATP, optionally, phosphorylation reaction stoppers, and; d) an appropriate detection system for quantifying the phosphorylation of the cNPK substrate. The invention also relates to an HTS-appropriate test system for detecting the activity of VASP phosphatases containing: e) at least one test compound; f) at least one appropriate VASP phosphatase substrate; g) at least one composition, which is to be incubated and which contains VASP phosphatase, and; h) an appropriate detection system for quantifying the dephosphorylation of the VASP phosphatase substrate. The described test systems can be used for locating compounds, which modulate the activity of a cNPK or of a VASP phosphatase, from chemical or natural substance libraries.
IPC 1-7
IPC 8 full level
C12Q 1/48 (2006.01)
CPC (source: EP US)
C12Q 1/485 (2013.01 - EP US); G01N 2333/91245 (2013.01 - EP US)
Citation (search report)
See references of WO 0196594A2
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
DOCDB simple family (publication)
WO 0196594 A2 20011220; WO 0196594 A3 20020516; AU 6395601 A 20011224; DE 10029210 A1 20020131; EP 1294926 A2 20030326; US 2003166005 A1 20030904
DOCDB simple family (application)
EP 0106621 W 20010612; AU 6395601 A 20010612; DE 10029210 A 20000614; EP 01938257 A 20010612; US 31109303 A 20030401