EP 1301798 A2 20030416 - MILD ENRICHMENT OF FOETAL CELLS FROM PERIPHERAL BLOOD AND USE THEREOF

Title (en)

MILD ENRICHMENT OF FOETAL CELLS FROM PERIPHERAL BLOOD AND USE THEREOF

Title (de)

SCHONENDE HOCHANREICHERUNG VON FETALEN ZELLEN AUS PERIPHEREM BLUT UND VERWENDUNG DERSELBEN

Title (fr)

ENRICHISSEMENT FORT ET MENAGE DE CELLULES EMBRYONNAIRES A PARTIR DE SANG PERIPHERIQUE ET UTILISATION DUDIT SANG

Publication

EP 1301798 A2 20030416 (DE)

Application

EP 01964843 A 20010717

Priority

DE 0102723 W 20010717
DE 10035433 A 20000720

Abstract (en)

[origin: WO0208751A2] The invention relates to a method for highly enriching foetal cells as products, whereby after a sample of maternal blood is taken and the centrifugation of blood fractions occurs, a cocktail of anti-bodies is added comprising anti-bodies anti.w, anti-bodies Anti-r, which bond specifically to transferrin-receptor-molecules as antigens of the surfaces of precursor of red blood cells containing a nucleus, and/or anti-bodies Anti-I, which bond specifically to antigen-I as antigens of the surfaces of precursor cells of red blood cells containing a nucleus and/or anti-bodies Anti I plus, which bond specifically to an intracellular structure of precursor foetal cells and/or the intracellular molecules of precursor foetal cells and/or membrane fragments of precursor foetal cells, and then the foetal cells which have been enriched 10<5> - 10<7> times are separated from the cell base.

[origin: WO0208751A2] The invention relates to a method for mildly enriching foetal cells, wherein after a sample of maternal blood is taken and the centrifugation thereof occurs, a cocktail of anti-bodies is added comprising anti-bodies anti-w, anti-bodies anti-r, anti-bodies anti-i and/or anti-bodies anti-i, then the foetal cells which have been enriched 10<5> - 10<7> times are separated from the cell set.

IPC 1-7

G01N 33/80; G01N 33/569; G01N 33/68; C12N 5/06

IPC 8 full level

G01N 33/48 (2006.01); A61K 35/12 (2006.01); A61K 48/00 (2006.01); C12N 5/00 (2006.01); C12N 5/06 (2006.01); C12N 5/08 (2006.01); G01N 33/569 (2006.01); G01N 33/80 (2006.01)

CPC (source: EP US)

G01N 33/56966 (2013.01 - EP US); G01N 33/80 (2013.01 - EP US)

Citation (search report)

See references of WO 0208751A2

Citation (examination)

LAN N. ET AL: "Ribozyme-mediated repair of sickle beta-globin mRNAs in erythrocyte precursors", SCIENCE, vol. 280, no. 5369, 5 June 1998 (1998-06-05), UNITED STATES, pages 1593 - 1596, XP002101539
OPIE TIM M. ET AL: "Cell-surface antigen expression in early and term gestation fetal hematopoietic progenitor cells", STEM CELLS, vol. 16, no. 5, 1998, MIAMISBURG, pages 343 - 348, XP009045821
EDDLEMAN KEITH A. ET AL: "Circulating hematopoietic stem cell populations in human fetuses: Implications for fetal gene therapy and alterations with in utero red cell transfusion", FETAL DIAGNOSIS AND THERAPY, vol. 11, no. 4, 1996, pages 231 - 240, XP009045823

Designated contracting state (EPC)

AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

DOCDB simple family (publication)

WO 0208751 A2 20020131; WO 0208751 A3 20020815; WO 0208751 A8 20020516; WO 0208751 A8 20020620; AU 8568301 A 20020205; DE 10035433 A1 20020207; DE 10035433 C2 20020718; EP 1301798 A2 20030416; JP 2004504818 A 20040219; US 2003180762 A1 20030925

DOCDB simple family (application)

DE 0102723 W 20010717; AU 8568301 A 20010717; DE 10035433 A 20000720; EP 01964843 A 20010717; JP 2002514394 A 20010717; US 33324503 A 20030506