EP 1565549 A1 20050824 - METHOD FOR THE PREPARATION, PURIFICATION AND DIFFERENTIATION OF NEUROSPHERES FROM MAMMALIAN STEM CELLS
Title (en)
METHOD FOR THE PREPARATION, PURIFICATION AND DIFFERENTIATION OF NEUROSPHERES FROM MAMMALIAN STEM CELLS
Title (de)
VERFAHREN ZUR HERSTELLUNG, REINIGUNG UND DIFFERENZIERUNG VON NEUROSPHûREN AUS SûUGERSTAMMZELLEN
Title (fr)
PROCEDE DE PREPARATION, DE PURIFICATION ET DE DIFFERENCIATION DE NEUROSPHERES ISSUES DE CELLULES SOUCHES DE MAMMIFERES
Publication
Application
Priority
- GB 0303395 W 20030804
- GB 0217981 A 20020802
Abstract (en)
[origin: WO2004013315A1] Method for the preparation of a neurosphere in the form of a cellular aggregate of neural progenitors capable of terminal differentiation to form neurons and optionally additionally glial cells comprising culturing a mammalian pluripotent stem cell line in the presence of differentiating agent to form one or more cell neurospheres and isolating intact neurospheres from single cells, small cell clumps and cell debris, characterised in that cells are isolated as intact neurospheres of purity in excess of 99%; method for dissociation of neurospheres thereby producing a population of single neural progenitor cells of high purity; substantially pure neurospheres and populations of single neural progenitor cells obtained thereby; compositions thereof; the use of neurospheres and single cell populations obtained by dissociation thereof or compositions thereof in in vitro differentiation, in in vitro research, modelling in tissue culture, pharmaceutical development including drug screening toxicological testing and therapeutic cell replacement strategies including transplantation, and the like; and a method for screening using the neurospheres, dissociated cells or composition.
IPC 1-7
IPC 8 full level
C12N 5/0797 (2010.01)
CPC (source: EP)
C12N 5/0623 (2013.01); C12N 2501/06 (2013.01); C12N 2501/385 (2013.01); C12N 2503/02 (2013.01); C12N 2506/02 (2013.01)
Citation (search report)
See references of WO 2004013315A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2004013315 A1 20040212; AU 2003255750 A1 20040223; EP 1565549 A1 20050824; GB 0217981 D0 20020911
DOCDB simple family (application)
GB 0303395 W 20030804; AU 2003255750 A 20030804; EP 03766486 A 20030804; GB 0217981 A 20020802