EP 2329017 A2 20110608 - NOVEL METHOD TO GENERATE MEGANUCLEASES WITH ALTERED CHARACTERISTICS
Title (en)
NOVEL METHOD TO GENERATE MEGANUCLEASES WITH ALTERED CHARACTERISTICS
Title (de)
NEUES VERFAHREN ZUR ERZEUGUNG VON MEGANUKLEASEN MIT VERÄNDERTEN EIGENSCHAFTEN
Title (fr)
NOUVELLE MÉTHODE POUR GÉNÉRER DES MÉGANUCLÉASES AYANT DES CARACTÉRISTIQUES MODIFIÉES
Publication
Application
Priority
- IB 2009000486 W 20090209
- IB 2008002999 W 20080804
- IB 2008003744 W 20081212
Abstract (en)
[origin: WO2010015899A2] Method to generate and select a meganuclease having at least two altered characteristics in comparison to a parent meganuclease, comprising the steps: a. constructing from a parent meganuclease, a first series of variants which differ from said parent meganuclease by at least one acid amino substitution; b. screening the variants from said first series of step a. and selecting those which have a first altered characteristic; c. constructing from the selected variants of step b. a second series of variants having a least one other amino acid substitution; d. screening the variants from said series of step b. and selecting those which have said first altered characteristic and a second altered characteristic. Polypeptide obtained from said method.
IPC 8 full level
C12N 9/22 (2006.01); C12N 15/09 (2006.01); C12N 15/90 (2006.01)
CPC (source: EP)
C12N 9/22 (2013.01); C12N 15/1058 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 2010015899A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA RS
DOCDB simple family (publication)
WO 2010015899 A2 20100211; WO 2010015899 A3 20100325; EP 2329017 A2 20110608
DOCDB simple family (application)
IB 2009000486 W 20090209; EP 09785836 A 20090209