EP 2888372 A1 20150701 - METHOD FOR PRODUCING VECTORS CONTAINING A GENE THAT CODES FOR AN ENZYME HAVING REDUCED OR SWITCHED-OFF FEEDBACK INHIBITION, AND USE THEREOF FOR PRODUCING AMINO ACIDS AND NUCLEOTIDES
Title (en)
METHOD FOR PRODUCING VECTORS CONTAINING A GENE THAT CODES FOR AN ENZYME HAVING REDUCED OR SWITCHED-OFF FEEDBACK INHIBITION, AND USE THEREOF FOR PRODUCING AMINO ACIDS AND NUCLEOTIDES
Title (de)
VERFAHREN ZUR HERSTELLUNG VON VEKTOREN ENTHALTEND EIN FÜR IN SEINER FEEDBACK-INHIBIERUNG GEMINDERTES ODER AUSGESCHALTETES ENZYM KODIERENDES GEN UND DEREN VERWENDUNG FÜR DIE HERSTELLUNG VON AMINOSÄUREN UND NUKLEOTIDEN
Title (fr)
PROCÉDÉ DE PRÉPARATION DE VECTEURS CONTENANT UN GÈNE CODANT UNE ENZYME, ATTÉNUÉ OU DÉSACTIVÉ POUR L'INHIBITION DE SON FEED-BACK, ET UTILISATION DESDITS VECTEURS POUR LA PRÉPARATION D'ACIDES AMINÉS ET DE NUCLÉOTIDES
Publication
Application
Priority
- DE 102012016716 A 20120822
- DE 2013000416 W 20130723
Abstract (en)
[origin: WO2014029376A1] The invention relates to a method for producing vectors containing a gene that codes for an enzyme having reduced or switched-off feedback inhibition, and use thereof for producing amino acids and nucleotides. According to the invention genes that code for feedback-inhibited enzymes are mutagenised in vitro, in a further step are ligated into vectors, in a further step each vector is transformed into a microorganism that contains a metabolite sensor, which at increased metabolite concentration causes the synthesis of a fluorescent protein, after which microorganisms having an increased or the highest fluorescence are selected, and the vectors that contain a gene coding for an enzyme having reduced or switched-off feedback inhibition are isolated from microorganisms having an increased or the highest fluorescence.
IPC 8 full level
C12Q 1/68 (2006.01); C12N 15/10 (2006.01); C12P 13/00 (2006.01)
CPC (source: EP US)
C12N 9/1217 (2013.01 - EP US); C12P 13/08 (2013.01 - EP US); C12P 19/34 (2013.01 - US); C12Y 207/02004 (2013.01 - EP US); Y02P 20/52 (2015.11 - US)
Citation (search report)
See references of WO 2014029376A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2014029376 A1 20140227; CN 104662169 A 20150527; DE 102012016716 A1 20140227; EP 2888372 A1 20150701; JP 2015529459 A 20151008; US 2015284760 A1 20151008; US 9644226 B2 20170509
DOCDB simple family (application)
DE 2013000416 W 20130723; CN 201380044415 A 20130723; DE 102012016716 A 20120822; EP 13753806 A 20130723; JP 2015527791 A 20130723; US 201314422394 A 20130723