EP 3250707 A1 20171206 - SELECTIVE DETECTION OF LACTIC AND/OR ACETIC BACTERIA
Title (en)
SELECTIVE DETECTION OF LACTIC AND/OR ACETIC BACTERIA
Title (de)
SELEKTIVER NACHWEIS VON MILCHSÄURE- UND/ODER ESSIGSÄUREBAKTERIEN
Title (fr)
DÉTECTION SÉLECTIVE DE BACTÉRIES LACTIQUES ET/OU ACÉTIQUES
Publication
Application
Priority
- FR 1500163 A 20150128
- FR 2016000011 W 20160125
Abstract (en)
[origin: WO2016120534A1] A method for detecting lactic acid and/or acetic acid bacteria in a food-processing matrix comprising a microbial flora (the microbial flora comprising a lactic acid and/or acetic acid bacterial flora) and a fungal flora, the bacterial flora containing an adenosine triphosphate of bacterial origin, the fungal flora containing an adenosine triphosphate of fungal origin, which comprises the following steps: - applying, to the matrix, an antifungal having an antifungal action which is lethal, at a first time limit, on the fungal flora, and an antibiotic action which is non-lethal, at a second time limit after the first time limit, on the bacterial flora, - detecting the microbial flora between the first time limit and the second time limit; in which the lethal antifungal action releases, into the matrix, for the first time limit, adenosine triphosphate of fungal origin and in which the microbial flora is detected between the first time limit and the second time limit by means of the following steps: - removing the free adenosine triphosphate from the matrix, then - applying a lysis agent to the matrix, in order to release from the matrix the adenosine triphosphate of bacterial origin, then - measuring the adenosine triphosphate released.
IPC 8 full level
C12Q 1/00 (2006.01); C12Q 1/04 (2006.01); C12Q 1/66 (2006.01); G01N 21/76 (2006.01); G01N 33/14 (2006.01)
CPC (source: CN EP US)
C12Q 1/008 (2013.01 - CN EP US); C12Q 1/04 (2013.01 - CN EP US); C12Q 1/66 (2013.01 - CN EP US); G01N 21/763 (2013.01 - CN EP US); G01N 33/14 (2013.01 - EP US); C12Q 2304/60 (2013.01 - US)
Citation (search report)
See references of WO 2016120534A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
FR 3031988 A1 20160729; FR 3031988 B1 20181019; CN 107406870 A 20171128; EP 3250707 A1 20171206; US 10415074 B2 20190917; US 2018037923 A1 20180208; WO 2016120534 A1 20160804
DOCDB simple family (application)
FR 1500163 A 20150128; CN 201680007461 A 20160125; EP 16707831 A 20160125; FR 2016000011 W 20160125; US 201615731735 A 20160125