EP 3519096 A4 20200617 - HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS FOR BACTERIAL COMMUNITY RELATIONSHIP DETERMINATION AND OTHER HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS
Title (en)
HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS FOR BACTERIAL COMMUNITY RELATIONSHIP DETERMINATION AND OTHER HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS
Title (de)
HOCHAUFLÖSENDE SYSTEME, KITS, VORRICHTUNGEN UND VERFAHREN ZUR BESTIMMUNG VON BEZIEHUNGEN VON BAKTERIENGEMEINSCHAFTEN UND ANDEREN MIKROBIOLOGISCHEN ANWENDUNGEN MIT HOHEM DURCHSATZ
Title (fr)
SYSTÈMES, KITS, APPAREIL ET PROCÉDÉS À HAUTE RÉSOLUTION POUR DÉTERMINATION DE RELATION COMMUNAUTAIRE BACTÉRIENNE ET AUTRES APPLICATIONS DE MICROBIOLOGIE À HAUT DÉBIT
Publication
Application
Priority
- US 201662400841 P 20160928
- US 2017054108 W 20170928
Abstract (en)
[origin: WO2018064385A1] A method is provided for analyzing a sample including a population of biological entities using at least one microfabricated device. A plurality of the microwells on the microfabricated device are each uniquely indexed, and loaded with a sample such that at least some microwells each include more than one cell of a biological entity. The microfabricated device was incubated at predetermined conditions, and a selected genetic material of the cells of the biological entities obtained from the incubation is amplified to obtaining amplicons. An aggregate of the amplicons are sequenced obtain sequencing data, based on which and the indexing of the microwells, an identification of the biological entities present in each of the plurality of microwells is obtained. Such identification can then be used to determine a relationship between different types of biological entities in the sample.
IPC 8 full level
B01L 3/02 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/689 (2018.01); C40B 20/02 (2006.01); C40B 60/14 (2006.01)
CPC (source: EP)
B01L 3/0244 (2013.01); B01L 3/5085 (2013.01); B01L 3/545 (2013.01); C12Q 1/6837 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/689 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00743 (2013.01); B01L 7/52 (2013.01); B01L 2200/025 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/12 (2013.01); B01L 2200/141 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/165 (2013.01); C40B 20/02 (2013.01)
Citation (search report)
- [Y] WO 2016138496 A1 20160901 - CELLULAR RES INC [US]
- [Y] US 2016010085 A1 20160114 - KWON SUNGHOON [KR], et al
- [Y] EP 2805769 A1 20141126 - EUROPEAN MOLECULAR BIOLOGY LAB EMBL [DE]
- [A] WO 2005010169 A2 20050203 - DIVERSA CORP [US], et al
- [A] LIANG MA ET AL: "Gene-targeted microfluidic cultivation validated by isolation of a gut bacterium listed in Human Microbiome Project's Most Wanted taxa", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 27, 25 June 2014 (2014-06-25), US, pages 9768 - 9773, XP055362053, ISSN: 0027-8424, DOI: 10.1073/pnas.1404753111
- [A] J.DAISY VANITHA ET AL: "Evaluation of microplate Alamar blue assay for drug susceptibility testing of Mycobacterium avium complex isolates", DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol. 49, no. 3, 1 July 2004 (2004-07-01), AMSTERDAM, NL, pages 179 - 182, XP055288738, ISSN: 0732-8893, DOI: 10.1016/j.diagmicrobio.2004.04.003
- See references of WO 2018064385A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2018064385 A1 20180405; AU 2017335897 A1 20190404; CA 3037631 A1 20180405; CN 109843435 A 20190604; EP 3519096 A1 20190807; EP 3519096 A4 20200617; IL 265540 A 20190530; JP 2019531730 A 20191107
DOCDB simple family (application)
US 2017054108 W 20170928; AU 2017335897 A 20170928; CA 3037631 A 20170928; CN 201780059848 A 20170928; EP 17857449 A 20170928; IL 26554019 A 20190321; JP 2019516965 A 20170928