EP 3759252 A1 20210106 - METHOD FOR THE SELECTIVE AMPLIFICATION OF NUCLEIC ACIDS AND KIT FOR CARRYING OUT SAME
Title (en)
METHOD FOR THE SELECTIVE AMPLIFICATION OF NUCLEIC ACIDS AND KIT FOR CARRYING OUT SAME
Title (de)
VERFAHREN ZUR SELEKTIVEN AMPLIFIKATION VON NUKLEINSÄUREN UND KIT ZU DESSEN DURCHFÜHRUNG
Title (fr)
PROCÉDÉ D'AMPLIFICATION SÉLECTIVE D'ACIDES NUCLÉIQUES ET KIT POUR METTRE EN OEUVRE CE PROCÉDÉ
Publication
Application
Priority
- EP 18158518 A 20180226
- EP 2019054545 W 20190225
Abstract (en)
[origin: WO2019162487A1] A method is disclosed for detecting an amplification of nucleic acids in which use is essentially made of the fact that a predefined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target-sequence-specific activator oligonucleotide. The target-sequence-specific activator oligonucleotide brings about the separation of de-novo synthesized complementary primer elongation products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective strand of the template. The complex thus formed, of a primer oligonucleotide and a template strand, can initiate a new primer elongation reaction. The primer elongation products thus formed, in turn, act again as templates, the result being an exponentially proceeding amplification reaction. In the method, a selective amplification is effected in that, for the first, second primer and activator oligonucleotide, variants are used which differ from the target sequence by at least one nucleotide.
IPC 8 full level
C12Q 1/6858 (2018.01)
CPC (source: EP US)
C12Q 1/6858 (2013.01 - EP US)
Citation (search report)
See references of WO 2019162487A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 3530756 A1 20190828; EP 3759252 A1 20210106; US 2021189479 A1 20210624; WO 2019162487 A1 20190829
DOCDB simple family (application)
EP 18158518 A 20180226; EP 19712689 A 20190225; EP 2019054545 W 20190225; US 201916975494 A 20190225