EP 3830294 A1 20210609 - REACTION MIXTURE, METHOD AND KIT FOR PERFORMING A QUANTITATIVE REAL-TIME PCR
Title (en)
REACTION MIXTURE, METHOD AND KIT FOR PERFORMING A QUANTITATIVE REAL-TIME PCR
Title (de)
REAKTIONSGEMISCH, VERFAHREN UND KIT ZUR DURCHFÜHRUNG EINER QUANTITATIVEN ECHTZEIT-PCR
Title (fr)
MÉLANGE RÉACTIONNEL, PROCÉDÉ ET KIT POUR RÉALISER UNE PCR QUANTITATIVE EN TEMPS RÉEL
Publication
Application
Priority
- DE 102018213027 A 20180803
- EP 2019066957 W 20190626
Abstract (en)
[origin: WO2020025222A1] A reaction mixture for providing a reaction batch for performing a quantitative real-time PCR contains at least one target DNA (11), which at least in parts corresponds to the DNA section being quantified, at least one reference DNA (12) of defined sequence and in a defined amount, at least two different fluorescent probes of different sequence which generate a signal at different wavelengths, primers, deoxynucleotides and a DNA polymerase. The target DNA (11) and the reference DNA (12) have the same primer binding sites (13, 14) and different probe binding sites (17, 18). At least one of the fluorescent probes is intended for binding to a section of the target DNA (11) outside the primer binding sites (13, 14) in the amplicon, and at least one of the fluorescent probes is intended for binding to a section of the reference DNA (12) outside the primer binding sites (13, 14) in the amplicon.
IPC 8 full level
C12Q 1/686 (2018.01)
CPC (source: EP US)
C12Q 1/686 (2013.01 - EP US)
C-Set (source: EP)
C12Q 1/686 + C12Q 2537/101 + C12Q 2545/101 + C12Q 2549/113 + C12Q 2561/113 + C12Q 2563/107
Citation (search report)
See references of WO 2020025222A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2020025222 A1 20200206; CN 112513291 A 20210316; DE 102018213027 A1 20200206; EP 3830294 A1 20210609; US 2021310047 A1 20211007
DOCDB simple family (application)
EP 2019066957 W 20190626; CN 201980051441 A 20190626; DE 102018213027 A 20180803; EP 19734358 A 20190626; US 201917264631 A 20190626