EP 3861108 A1 20210811 - METHODS AND COMPOSITIONS FOR INCREASING CAPPING EFFICIENCY OF TRANSCRIBED RNA
Title (en)
METHODS AND COMPOSITIONS FOR INCREASING CAPPING EFFICIENCY OF TRANSCRIBED RNA
Title (de)
VERFAHREN UND ZUSAMMENSETZUNGEN ZUR ERHÖHUNG DER VERKAPPUNGSEFFIZIENZ VON TRANSKRIBIERTER RNA
Title (fr)
PROCÉDÉS ET COMPOSITIONS POUR AUGMENTER L'EFFICACITÉ DE COIFFAGE D'UN ARN TRANSCRIT
Publication
Application
Priority
- US 201862741111 P 20181004
- US 2019054715 W 20191004
Abstract (en)
[origin: WO2020072914A1] Methods and compositions for capping RNA in an in vitro transcription (IVT) mixture are provided that include a thermostable RNA polymerase variant and a cap analog such that when a DNA template is added to the mixture, and the mixture is then incubated under conditions for in vitro transcription, capped RNA is produced. The methods describe high capping efficiencies at elevated temperatures as well as at standard temperatures for IVT. The RNA polymerase used is a thermostable variant of the T7 RNA polymerase, named polymerase M20, which comprises substitutions at positions 388 and 567, in particular D388E and V567P.
IPC 8 full level
C12N 9/12 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP)
C12N 9/1247 (2013.01); C12N 15/09 (2013.01); C12Y 207/07006 (2013.01); C12N 2310/317 (2013.01)
Citation (search report)
See references of WO 2020072914A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
WO 2020072914 A1 20200409; AU 2019355177 A1 20210506; CA 3114892 A1 20200409; CN 113166737 A 20210723; EP 3861108 A1 20210811
DOCDB simple family (application)
US 2019054715 W 20191004; AU 2019355177 A 20191004; CA 3114892 A 20191004; CN 201980076560 A 20191004; EP 19839450 A 20191004