EP 4077724 A1 20221026 - METHOD AND DEVICE FOR DETERMINING THE NUMBER OF COPIES OF A DNA SEQUENCE THAT IS PRESENT IN A FLUID
Title (en)
METHOD AND DEVICE FOR DETERMINING THE NUMBER OF COPIES OF A DNA SEQUENCE THAT IS PRESENT IN A FLUID
Title (de)
VERFAHREN UND VORRICHTUNG ZUM ERMITTELN EINER IN EINEM FLUID ENTHALTENEN KOPIENANZAHL EINER DNA-SEQUENZ
Title (fr)
PROCÉDÉ ET DISPOSITIF POUR DÉTERMINER LE NOMBRE DE COPIES D'UNE SÉQUENCE D'ADN PRÉSENTES DANS UN FLUIDE
Publication
Application
Priority
- DE 102019220020 A 20191218
- EP 2020086752 W 20201217
Abstract (en)
[origin: CA3161507A1] The invention relates to a method (100) for determining the number of copies of (300) of a DNA sequence that is present in a fluid, wherein the method (100) comprises a division step (102), a setting up step (105), an identification step (110) and an evaluation step (115). In the division step (102), at least some of the fluid is divided into at least two compartments. In the setting up step (105), a reaction condition is set up for the fluid divided into the at least two compartments in order to allow a reaction in each of the at least two compartments and to obtain a reaction result in each case. In the identification step (110), a signal, for example an optical signal, is identified that represents the reaction results of the reactions that may have taken place in the compartments. In the evaluation step (115), the optical signal is evaluated in order to determine the number of copies , taking into account the statistical distribution of the copies across the compartments and taking into account a reaction-specific detection probability function which indicates the probability that an amplification reaction has taken place in a compartment, according to the number of copies initially present in the compartment.
IPC 8 full level
C12Q 1/6851 (2018.01)
CPC (source: EP KR US)
C12Q 1/6851 (2013.01 - EP KR US); C12Q 1/6848 (2013.01 - US); C12Q 2537/16 (2013.01 - KR); C12Q 2537/165 (2013.01 - KR); C12Q 2545/114 (2013.01 - KR); C12Q 2600/16 (2013.01 - US)
C-Set (source: EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
DE 102019220020 A1 20210624; CA 3161507 A1 20210624; CN 114787382 A 20220722; EP 4077724 A1 20221026; JP 2023507597 A 20230224; JP 7441315 B2 20240229; KR 20220118468 A 20220825; US 2023029306 A1 20230126; WO 2021122979 A1 20210624
DOCDB simple family (application)
DE 102019220020 A 20191218; CA 3161507 A 20201217; CN 202080086669 A 20201217; EP 2020086752 W 20201217; EP 20835768 A 20201217; JP 2022537635 A 20201217; KR 20227023934 A 20201217; US 202017757466 A 20201217