EP 4278003 A1 20231122 - LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) ON A SOLID-PHASE MEDIUM
Title (en)
LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) ON A SOLID-PHASE MEDIUM
Title (de)
LOOP-VERMITTELTE ISOTHERMALE AMPLIFIKATION (LAMP) AUF EINEM FESTPHASENMEDIUM
Title (fr)
AMPLIFICATION ISOTHERME INDUITE PAR BOUCLE (LAMP) SUR UN MILIEU EN PHASE SOLIDE
Publication
Application
Priority
- US 202163138321 P 20210115
- US 2022012640 W 20220115
Abstract (en)
[origin: WO2022155550A1] The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.
IPC 8 full level
C12Q 1/6806 (2018.01); C12Q 1/6848 (2018.01)
CPC (source: EP)
C12Q 1/6806 (2013.01); C12Q 1/6848 (2013.01)
C-Set (source: EP)
Citation (search report)
See references of WO 2022155550A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
WO 2022155550 A1 20220721; AR 124664 A1 20230419; EP 4278003 A1 20231122; TW 202246524 A 20221201
DOCDB simple family (application)
US 2022012640 W 20220115; AR P220100088 A 20220117; EP 22704038 A 20220115; TW 111101760 A 20220114